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This assay uses a standard laboratory fluorescence microplate reader as the detection instrument and was optimized for various experimental conditions such as cell density, actin filament staining reagent, staining buffer, and cell culture conditions. We developed a 96-well actin polymerization assay that can process 1,000 to 1,500 samples a day. Conventionally, actin polymerization is measured by fluorescence microscopy or flow cytometry, which are either of low throughput or in need of special instruments. Cells undergo cytoskeletal rearrangement prior to chemotaxis, and therefore actin polymerization can be used as a surrogate readout more proximal to chemokine receptor activation than chemotaxis. Therefore, disrupting CXCL13-induced chemotaxis may be a fruitful approach for developing therapeutics in treating these diseases. CXCR5 and its ligand CXCL13 participate in ectopic germinal center formation at the inflammatory sites of multiple immune diseases such as rheumatoid arthritis, multiple sclerosis, and Sjogren’s syndrome. The chemokine receptor CXCR5 is predominantly expressed on mature B cells and follicular T-helper cells. Thus, the higher transfection efficiency is attributed to the greater biocompatibility and flexibility of the amino acid/peptide-substituted gemini surfactants and demonstrates the feasibility of using amino acid-substituted gemini surfactants as gene carriers for the treatment of diseases affecting epithelial tissue. An interesting result revealed by the dye exclusion assay suggests a more balanced protection of the DNA by the glycine and glycyl-lysine substituted compounds. An overall low toxicity is observed for all complexes with no significant difference among substituted and unsubstituted compounds. In this study, we evaluated the cytotoxicity of P/G/L nanoparticles and explored the relationship between transfection efficiency/toxicity and their physicochemical characteristics (such as size, binding properties, etc.). Plasmid/gemini/lipid (P/G/L) nanoparticles formulated using these novel compounds achieved higher gene expression than the nanoparticles containing the parent unsubstituted compound. These compounds were synthesized and tested in rabbit epithelial cells using a model plasmid and a helper lipid. Gxps xps to pdf series#Recently, we have designed and developed a series of novel amino acid-substituted gemini surfactants with the general chemical formula C12H25(CH3)2N+- (CH2)3-N(AA)-(CH2)3-N+(CH3)2-C12H25 (AA= glycine, lysine, glycyl-lysine and, lysyl-lysine). To capitalize on the true potential of gene therapy, there is a need to develop better delivery systems that can protect these therapeutic biomolecules and deliver them safely to the target sites. Gene based therapy represents an important advance in the treatment of diseases that heretofore have had either no treatment or cure. High-Content Screening with AgileOptix Technology.PROTEIN DETECTION, QUANTITATION, ANALYSIS.NUCLEIC ACID (DNA/RNA) DETECTION & ANALYSIS.SpectraTest Validation Plates and Recertification. ![]() Gxps xps to pdf software#GXP SOFTWARE INSTALLATION AND VALIDATION.HIGH-THROUGHPUT, HIGH CONTENT SCREENING.you just have to select which file should be converted we will convert it for you.COVID-19 RESPONSE - We are committed to supporting our scientific community during this pandemic. ![]() Gxps xps to pdf registration#
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